An assessment of plant growth regulators on asymbiotic development and germination of immature embryos of Beclardia Macrostachya (orchidaceae)
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Accepted: 4 July 2011
Authors
Beclardia macrostahya is one of the rarest orchids in Mauritius. In vitro techniques are being used for mass propagating this orchid for subsequent restoration programs. Successful asymbiotic germination of Beclardia macrostachya was obtained through embryo rescue. Modified half MS media supplemented with 10% coconut milk was used as basal culture media and the effect of plant growth regulators at different concentrations on embryo development was assessed through qualitative and quantitative parameters. Diameter of embryos, length of protocorm-like bodies (PLBs) and length of developing shoots were calculated using digital photography. Maximal growth was obtained in treatments without any plant growth regulators and 0.5mg/l N6 Benzyl Amino Purine (BAP). Higher levels of Thiadurazon/TDZ (0.3mg/l) and BAP (1.0 mg/l) though they stimulated embryo development faster, yielded higher level of necrosis later. The results also suggest that plant growth regulator treatments that stimulate fastest embryo development from immature embryos/ovules need not be reliable for further development to PLB and plantlet regeneration.
Downloads
Citations
